Journal: Redox Biology

Article Title: Peroxiredoxin Ⅲ safeguards cardiac function against doxorubicin by regulating mitochondrial quality control via H 2 O 2 detoxification

doi: 10.1016/j.redox.2026.104176

Figure Lengend Snippet: PrxⅢ regulates oxidative damage and mitochondrial bioenergetics in DOX-treated cardiomyocytes. (A) Cells were transduced with Ad-PrxⅢ or Ad-Stuffer for 24 h and then exposed to 1 μM DOX for 12 h. Cells were labeled with 5 μM 10-NAO and relative fluorescence intensity (RFI) was analyzed by flow cytometer. Representative histograms and quantification are shown. (B) Cells were treated with 1 μM DOX for 12 h and labeled with 10 μM Rho-123 to assess mitochondrial membrane potential ( ΔΨ m ). The percentage of cells with low ΔΨ m was quantified by flow cytometry. Representative histograms and quantification are shown. (C) Cells were transduced with Ad-PrxⅢ or Ad-Stuffer for 24 h and then exposed to 1 μM DOX for 12 h. Representative bar graph showing relative ATP concentration normalized to protein contents. (D-H) Cells were transduced with Ad-PrxⅢ or Ad-Stuffer for 24 h and then exposed to 1 μM DOX for 12 h. (D) Representative tracing of the oxygen consumption rate (OCR). Arrows indicate time points when cells were treated with oligomycin, FCCP, and rotenone plus antimycin A (Rot/AA), respectively. The OCR is calculated as (E) basal respiration, (F) maximal respiration, (G) spare respiration, and (H) proton-leak. All data are expressed as mean ± S.D. (n = 3-5). Statistical significance was determined using two-way ANOVA followed by Bonferroni's post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: DOX was kindly provided from Donga-ST Co., Ltd. (Seoul, Republic of Korea); Fetal Bovine Serum (FBS) and antibiotic-antimycotic (A/A) were from Gibco (NY, USA); Dimethyl sulfoxide (DMSO), poly- l -lysine, puromycin, and bafilomycin A1 (BafA1) were from Sigma-Aldrich (St. Louis, MO, USA); 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-1) reagent was purchased from Roche Applied Science (Basel, Switzerland); Lipofectamine 2000, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), Rhodamine-123 (Rho-123), Mitotracker Red CMX, Lysosensor Green DND-189, DQ-BSA and 7-Aminoactinomycin D (7-AAD) were purchased from Invitrogen (CA, USA); 10-N-nonylacridine orange (10-NAO) was purchased from Molecular Probes (Eugene, OR, USA); Mitochondria peroxy yellow-1 (MitoPY-1) was from Tocris Biosciences (Bristol, UK); Magic Red kit was from ImmunoChemistry Technologies (Davis, CA, USA); paraformaldehyde (PFA), 4′,6-diamidno-2-phenylindole (DAPI) were from Thermo Fisher Scientific (Waltham, MA, USA); Adenosine triphosphate (ATP) and cell viability ATP assay kit was from Promega (Madison, WI, USA); Seahorse XF cell Mitostress test kit, Seahorse XF DMEM Medium, and Seahorse XF Calibrant Solution was from Agilent (Santa Clara, CA, USA); Elamipretide (SS-31) was from Biorbyt Ltd. (Cambridge, UK); Mitoquinone (MitoQ) and Visomitin (SKQ1) were from MedChemExpress (Monmouth junction, NJ, USA); Mouse Troponin I ELISA kit was from Abcam (Cambridge, UK).

Techniques: Transduction, Labeling, Fluorescence, Flow Cytometry, Membrane, Concentration Assay

Journal: Frontiers in Plant Science

Article Title: Characterizing the plant functional traits of coffee agroecosystems in Indonesia

doi: 10.3389/fpls.2025.1743035

Figure Lengend Snippet: Leaf structural, physiological, and chemical traits across different sites and seasons. (a) leaf mass per area (LMA), (b) leaf area, (c) leaf thickness, (d) light-saturated photosynthetic rate per unit leaf dry mass (A mass ), (e) maximum carboxylation rate (V cmax ), (f) maximum electron transport rate (J max ), (g) leaf C:N, (h) leaf nitrogen content, and (i) leaf phosphorus content. Different colors of the violin plot refer to different sites, and the different patterns refer to different seasons (solid = dry season, white stripe = wet season). The black dot inside the violin plot refers to the mean value, and the error bar represents the confidence interval. T‐test shows the significant difference between the wet and dry seasons within each site, with p‐value< 0.001(****), <0.01(***), <0.05(**), <0.1(*).

Article Snippet: Leaf carbon (C) content, leaf nitrogen (N) content, C isotope (δ 13 C), and N isotope (δ 15 N) were analysed using the Elemental Analyzer-Isotope Ratio Mass Spectrometry (EA-IRMS) technique (EA: EuroVector, model EA3000; IRMS: Nu Instruments, model Perspective).

Techniques:

Journal: Frontiers in Plant Science

Article Title: Characterizing the plant functional traits of coffee agroecosystems in Indonesia

doi: 10.3389/fpls.2025.1743035

Figure Lengend Snippet: Pairwise trait relationship of (a) Leaf nitrogen (N) content vs. light-saturated photosynthetic rate per unit dry mass (A mass ), (b) leaf mass per area (LMA) vs. A mass , (c) LMA vs. leaf N content. Grey datapoints are observations obtained from the TRY database, and the blue triangle data points are observations from this study aggregated across sites and seasons.

Article Snippet: Leaf carbon (C) content, leaf nitrogen (N) content, C isotope (δ 13 C), and N isotope (δ 15 N) were analysed using the Elemental Analyzer-Isotope Ratio Mass Spectrometry (EA-IRMS) technique (EA: EuroVector, model EA3000; IRMS: Nu Instruments, model Perspective).

Techniques: